Nitrate reductase clone and expression

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August undefined, 2024

Webb8 aug. 1991 · A 1.2 kb cDNA (pCVNR1) was isolated from a lambda gt11 expression library produced from polyadenylated RNA extracted from nitrate-grown Chlorella cells. pCVNR1 hybridized to a 3.5 kb mRNA transcript that was nitrate-inducible and absent from ammonium-grown cells. WebbThe molecular mass of the encoded protein was predicted to be 99.5 kDa, with an isoelectric point of 8.31. This protein shares common structural features with NRs from …

The nitrate reductase gene-switch: a system for regulated expression …

WebbThe genes encoding assimilatory nitrite reductase from Acidovorax wautersii QZ-4 were cloned and over-expressed in Escherichia coli. The molecular mass of over-expressed nitrite reductase was 91.75 kDa and 12.40 kDa for large subunit NirB and small subunit NirD, respectively. Webb13 apr. 2024 · Expression of the following genes related to nitrogen metabolism was determined: NR genes OsNia1 (LOC4345795) and OsNia2 (LOC4330867), nitrate transporter protein gene OsNrt 2.4 (LOC4336175), nitrite reductase gene OsNiR (LOC4330835), and glutamate synthase gene OsNADH-GOGAT (LOC4324398). grimmykitty

Chapter 1 Introduction Cloning, sequence analysis and studies …

Webb15 juli 2024 · In order to express nitrite reductase, gene nirB was cloned into pET-28a-Acma vector and nirD was cloned into pETDuet-1 vector, and all of them were over-expressed in E.coli BL21 (DE3) (Fig. 3). When pET-28a-NirB-Acma and pETDuet-NirD were co-expressed in soluble form in E. coli BL21 (DE3), the Acma-tagged NirB protein … WebbCloning and expression of distinct nitrite reductases in tobacco leaves and roots. Three tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR … WebbAbstract. Nitrate reductase (NR) is a key enzyme that catalyzes the first step in plants nitrogen metabolization. A cDNA of a NR gene from non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) cultivar ‘Suzhouqing’ was isolated by RT-PCR and (5′/3′)-RACE techniques. The full-length cDNA sequence of 3049 bp contained an ... grimmy inkitt

Cloning and expression of distinct nitrite reductases in ... - Springer

Cloning and expression analysis of a nitrate reductase gene …

Webb1 nov. 2003 · Expression of YNR1 under control of the MOX promoter in the presence of ammonium or nitrate. H. polymorpha strain bearing the YNR1 -coding region fused to the MOX gene promoter was grown in ammonium-containing medium. WebbThree tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR cDNA as a probe. Sequence analysis and Southern blot hybridization revealed four … grimm villainsWebbThe nitrate transporter polypeptide (NRT2) is characterised by 12 transmembrane domains and presents both a long putative intracellular loop and a short C-terminal … grimm style tattoos

"Webb13 apr. 2024 · Our study shows that expression of NPF8.4 and most ... glycolate oxidase; HPR1, hydroxypyruvate reductase 1; 2OG, 2 ... Table 4), and cloned into the oocyte expression vector ... " - Nitrate reductase clone and expression

Nitrate reductase clone and expression

Cloning and expression of distinct nitrite reductases in tobacco …

Webb14 apr. 2024 · Low-light intensity affects plant growth and development and, finally, causes a decrease in yield and quality. There is a need for improved cropping strategies to solve the problem. We previously demonstrated that moderate ammonium:nitrate ratio (NH4+:NO3−) mitigated the adverse effect caused by low-light stress, although … WebbCloning and expression analysis of a nitrate reductase gene related to somatic embryogenesis in Gossypium hirsutum Jie Liu1, Gaiying Han1, Yan Zhang1, Xingfen …

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WebbThe term dissimilatory N-oxide reduction is also used to indicate that the products are lost to the environment; this distinguishes the reactions from the assimilatory reductions used by plants and bacteria to incorporate nitrogen into biomass. 1.1.1The importance of … Webb31 mars 2024 · Gene Expression. nirA, ... nirA d-106 9 an allele conferring nitrogen metabolite derepression of nitrate and nitrite reductase activity, includes two transitions, changing a glutamic acid to a lysine and a valine to an alanine, situated between a basic and a proline-rich region of the protein.

Webb28 jan. 2010 · Nitrate reductase is a high-molecular-weight complex protein with three prosthetic groups, FAD, heme (cytochrome b 557 ), and Mo-pterin, which uses NADH … Webb6 okt. 2024 · E. coli DH5α and E. coli Rosetta-gami (Novagen) were used as cloning and expression hosts ... D. Potential use of nitrate reductase as a biomarker for the …

Webbby nitrate- or ammonium-derived metabolites. In addi- tion, the expression of the root NiR gene was detectable in leaves of a tobacco nitrate reductase (NR)-deficient mutant. … Webb1 juni 1992 · cDNA Clones for Corn Leaf NADH:Nitrate Reductase and Chloroplast NAD (P):Glyceraldehyde-3-Phosphate Dehydrogenase : Characterization of the Clones and Analysis of the Expression of the Genes in Leaves as Influenced by Nitrate in the Light and Dark. Gowri G , Campbell WH Plant Physiol, (3):792-798 1989 MED: 16666879

WebbNitrate is the major source of nitrogen taken from the soil by higher plants but requires reduction to ammonia prior to incorporation into amino acids. The first …

Webb24 okt. 2014 · Kinetics of nirS expression (cytochrome cd 1 nitrite reductase) in Pseudomonas stutzeri during the transition from aerobic respiration to denitrification: evidence for a denitrification-specific nitrate- and nitrite-responsive regulatory system. J Bacteriol. 181, 161–166. [PMC free article] [Google Scholar] grimm saison 7Webb14 okt. 2024 · The gene encoding the assimilation of nitrite reductase in B. cereus LJ01 was cloned and overexpressed in E. coli. The purified recombinant LJ01-NiR has a … grimm tattoo kansas cityWebbIn order to study the role of nitrate transporter (NRT) in the uptake and transport of nitrate in tobacco, a nitrate transporter gene encoding 612 amino acids with a total length of 1 … grimvuttyWebbMycorrhizal symbioses are a rule in nature and may have been crucial in plant and fungal evolution. Ecto_mycorrhizas are mutualistic interactions between tree grim sensitivity valorantWebbNitrate reductase-deficient mt− cells were cotransformed with a plasmid containing the nitrate reductase gene (Kindle et al. 1989; Tam and Lefebvre 1993) ... vegetatively growing samples from 120 of the clones were assayed for expression of HSV-tagged GSP1 using immunoblotting with an anti-HSV antibody. grimontia hollisaeWebb31 jan. 2024 · Nitrate reduction in E. coli is performed by the terminal reductases NarGHJI, NarZYWV, and NapFDAGHBC, the first two being membrane-bound (respiratory reductases) and the latter, periplasmic (dissimilatory reductase). grímsvötn vulkaanWebbAbstract. A cDNA corresponding to the nitrate reductase (NR) gene from Dunaliella salina was isolated by RT-PCR and (5′/3′)-RACE techniques. The full-length cDNA sequence of 3,694 bp contained an open reading frame of 2,703 bp encoding 900 amino acids, a 5′-untranslated region of 151 bp and a 3′-untranslated sequence of 840 bp with a ... grimy kitty